![]() ![]() developed with Pierce ECL Western Blotting Substrate (Thermo Fisher. This reaction produces a prolonged chemiluminescence which can be. Pierce ECL Western Blotting Substrate enables the detection of picogram amounts of antigen. ![]() Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare. Primary antibodies used in Western blot analysis were as follows: polyclonal anti. Thermo Scientific Pierce ECL Western Blotting Substrate is an, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.īecause the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity.
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